• Home
  • Issues
    • Current
    • All Issues
  • About
    • Aims and Scope
      Editorial Board
      Indexing
      Sources of Financing
      Ethics & Policies
      Publication Ethics Conflict of Interest Open Access Policy Archiving Complaints Policy Privacy Statement Corrections and Retractions Academic Integrity Generative AI Policy
      For Authors
      Terms of Publication Formatting Guidelines Peer Review Process Article Processing Charges License Agreement
  • Submission
  • Contacts
en
  • Українська

Ukrainian Black Sea Region Agrarian Science

  • Submit an article
  • Home
  • Issues
    • Current
    • All Issues
  • About
    • Aims and Scope
    • Editorial Board
    • Indexing
    • Sources of Financing
  • For Authors
    • Submission
    • Terms of Publication
    • Formatting Guidelines
    • Peer Review Process
    • Article Processing Charges
    • License Agreement
  • Ethics & Policies
    • Publication Ethics
    • Conflict of Interest
    • Open Access Policy
    • Archiving
    • Complaints Policy
    • Privacy Statement
    • Corrections and Retractions
    • Academic Integrity Generative AI Policy
  • Contacts

Article

  • Read article
  • Download article

Received 23.10.2025

Revised 01.05.2026

Accepted 26.05.2026

Published 30.06.2026

Retrieved from Volume 30, No. 2, 2026

Pages 41 -48

  • 33 Views

Suggested citation

Ivashchenko, I., Vitenko, V., Mamchur, V., & Kozhebash, A. (2026). Pre-sowing preparation and agrotechnics of sowing as factors of soil germination seeds of Thuja plicata Don. ex D. Donn. Ukrainian Black Sea Region Agrarian Science, 30(2), 41-48. https://doi.org/10.56407/bs.agrarian/2.2026.41

Pre-sowing preparation and agrotechnics of sowing as factors of soil germination seeds of Thuja plicata Don. ex D. Donn

Iryna Ivashchenko Volodymyr Vitenko Valentina Mamchur Anastasia Kozhebash

Abstract

This study aimed to determine the optimal conditions for pre-sowing seed preparation and sowing depth to improve soil germination of Thuja plicata. The study was conducted using Thuja plicata seed material collected in the National Dendrological Park “Sofiyivka” of the NAS of Ukraine to evaluate seed quality, germination, and the effects of different pre-sowing treatments using standard methods. The results demonstrated significant effects of collection time, germination temperature, pre-sowing treatments, and sowing depth on seed performance. Seeds collected in autumn from standing trees showed higher germination energy (35.4-39.2%) and laboratory germination (44.4-48.7%) compared with seeds collected in winter from the snow surface, which demonstrated lower values of germination energy (27.1-31.6%) and laboratory germination (34.3-39.1%). Germination at +25 °C increased germination energy and final laboratory germination compared with room temperature (18-20 °C). Soil germination was strongly influenced by sowing depth and pre-sowing treatments. The highest soil germination (43.2 ± 2.1%) was recorded at a sowing depth of 0.5 cm after 30-day snow stratification, which exceeded the control treatment (32.7 ± 1.8%). Water soaking for 12 hours also improved germination, reaching 39.3 ± 1.9% at the same sowing depth. Increasing sowing depth to 2.0 cm resulted in a sharp decline in soil germination, reaching 10.1 ± 1.2% after snow stratification and complete absence of seedlings in the control treatment. Snow stratification also accelerated seedling emergence, reducing the time to first emergence from 14 days in the control to 12 days. These findings indicate that autumn seed harvesting, 30-day snow stratification, and shallow sowing (0.5-1.0 cm) provide the most favourable conditions for successful germination and seedling establishment of Thuja plicata

Keywords:

introduced taxon; conifer reproduction; stratification methods; adaptive capacity; planting material production

References

  1. Aldana, J., Kope, H., & Hawkins, B. (2023). Western redcedar: Natural history and pathosystems, with emphasis on cedar leaf biight. Canadian Journal of Plant Pathology, 45(4), 377-390. doi: 10.1080/07060661.2023.2198489.
  2. Antos, J., Filipescu, C., & Negrave, R. (2016). Ecology of western redcedar (Thuja plicata): Implications for management of a high-value multiple-use resource. Forest Ecology and Management, 375, 211-222. doi: 10.1016/foreco.2016.05.043.
  3. Awasthi, P. (2023). Review on dormancy, causes, uses, and measures of overcoming it. Science Heritage Journal, 7(2), 56-70. doi: 10.26480/gws.02.2023.57.60.
  4. Baskin, C., & Baskin, J. (2014). Seeds: Ecology, biogeography, and evolution of dormancy and germination (2nd ed). San Diego: Academic Press.
  5. Botulinski, R. (2021). Plant propagation protocol for Thuja plicata ESRM 412 – native plant production. Retrieved from https://courses.washington.edu/esrm412/protocols/2021/THPL.pdf
  6. Debryniuk, Yu., Kalinin, M., Huz, M., & Shabliy, I. (1998). Forest seed production. Lviv: Svit.
  7. Ferras, A., Vanier, P., Marcora, P., Tecco, P., Funes, G., Giogis, M., Gallara, F., & Zeballos, S. (2025). Towards the construction of a seed traits database for restoration: Effects of light, temperature and seed storage on germination. Forest Ecology and Management, 577, article number 122390. doi: 10.1016/j.foreco.2024.122390.
  8. Guillardín, L., Glover, E., Kerr, G., & MacKay, J. (2025). Genomic exploration of Thuja plicata indicates no loss of diversity from adults to offspring in UK woodlands. Tree Genetics & Genomes, 21, article number 5. doi: 10.1007/s11295-025-01689-7.
  9. Hawkins, B., Guest, H., & Kolotelo, D. (2003). Freezing tolerance of conifer seeds and germinants. Tree Physiology, 23(18), 1237-1246. doi: 10.1093/treephys/23.18.1237.
  10. Khadduri, N. (2007). Greenhouse germination trials of pelletized Western Redcedar and Red Alder seeds. USDA Forest Service Proceedings, 50, 15-19.
  11. Kohinoor, B., Nazmul, H., & Mashura, S. (2024). Selective biotic stressors’ action on seed germination: A review. Plant Science, 346, article number 112156. doi: 10.1016/j.plantsci.2024.112156.
  12. Marynych, I. (1998). Growth and development of North American conifers under the conditions of the forest-steppe zone of Ukraine. Scientific Bulletin of the Ukrainian State Forestry University, 9(2), 63-66.
  13. Owens, J., & Molder, M. (2011). Sexual reproduction in western red cedar (Thuja plicata). Canadian Journal of Botany, 58(12), 1376-1393. doi: 10.1139/b80-169.
  14. Schopmeyer, C. (1974). Seeds of woody plants in the United States. Washington: USDA Forest Service.
  15. Shlapak, V., & Vitenko, V. (2019). Biological and ecological peculiarities of Maclura Рomifera (rafin.) Schneid: Introduction, biology, ecology and commercial use. Proceedings of the Forestry Academy of Sciences of Ukraine, 8, 53-58.
  16. Smahliuk, K. (1976). Introduced coniferous forest-forming species. Uzhhorod: Karpaty.
  17. Song, Y., Li, X., Zhang, M., Xia, G., & Xiong, C. (2023). Effect of cold stratification on the temperature range for germination of Pinus koraiensis. Journal of Forestry Research, 34, 1121-1130. doi: 10.1007/s11676-022-01540-y.
  18. Terskikh, V.V., Zeng, Y., Feurtado, J.A., Giblin, M., Abrams, S.R., & Kermode, A.R. (2008). Deterioration of western redcedar (Thuja plicata) seeds: Protein oxidation and in vivo NMR monitoring of storage oils. Journal of Experimental Botany, 59(4), 765-777. doi: 10.1093/jxb/erm357.
  19. Wang, J., Bu, Z., Poschlod, P., Yusup, S., Zhang, J., & Zhang, Z. (2024). Seed dormancy types and germination response of 15 plant species in temperate montane peatlands. Ecology and Evolution, 14, article number e11243. doi: 10.1002/ece3.11671.
  20. Wang, J., Qian, H., & Klinka, K. (1994). Growth of Thuja plicata seedlings along a light gradient. Canadian Journal of Botany, 72(12), 1749-1757. doi: 10.1139/b94-215.
  21. Young, J., & Young, C. (1993). Seeds of woody plants in North America. Portland: Dioscorides Press.
Share
Facebook
Twitter
LinkedIn
Email
Telegram
Viber
WhatsApp

Address
54020, Ukraine, Mykolaiv,
9 Georgiy Gongadze Str.


Email
ubsras@bsagriculture.com.ua

Main information
  • Aims and Scope
  • Indexing
  • Terms of Publication
  • Editorial Board
  • Publication Ethics
Additional information
  • Complaints Policy
  • Peer Review Process
  • Open Access Policy
  • Academic Integrity Generative AI Policy
  • Archiving