• Home
  • Issues
    • Current
    • All Issues
  • About
    • Aims and Scope
      Editorial Board
      Indexing
      Sources of Financing
      Ethics & Policies
      Publication Ethics Conflict of Interest Open Access Policy Archiving Complaints Policy Privacy Statement Corrections and Retractions Academic Integrity Generative AI Policy
      For Authors
      Terms of Publication Formatting Guidelines Peer Review Process Article Processing Charges License Agreement
  • Submission
  • Contacts
en
  • Українська

Ukrainian Black Sea Region Agrarian Science

  • Submit an article
  • Home
  • Issues
    • Current
    • All Issues
  • About
    • Aims and Scope
    • Editorial Board
    • Indexing
    • Sources of Financing
  • For Authors
    • Submission
    • Terms of Publication
    • Formatting Guidelines
    • Peer Review Process
    • Article Processing Charges
    • License Agreement
  • Ethics & Policies
    • Publication Ethics
    • Conflict of Interest
    • Open Access Policy
    • Archiving
    • Complaints Policy
    • Privacy Statement
    • Corrections and Retractions
    • Academic Integrity Generative AI Policy
  • Contacts

Article

  • Read article
  • Download article

Received 06.11.2025

Revised 02.03.2026

Accepted 31.03.2026

Published 13.04.2026

Retrieved from Vol. 30, No. 1, 2026

Pages 19 -34

  • 208 Views

Suggested citation

Naidich, O., Bondar, А., & Brodovskyi, V. (2026). Modern biotechnological solutions in the prevention and control of coccidiosis in piglets on pig farms in Southern Ukraine. Ukrainian Black Sea Region Agrarian Science, 30(1), 19-34. https://doi.org/10.56407/bs.agrarian/1.2026.19

Modern biotechnological solutions in the prevention and control of coccidiosis in piglets on pig farms in Southern Ukraine

Olga Naidich Alla Bondar Viacheslav Brodovskyi

Abstract

The aim was to theoretically substantiate and develop practical recommendations for a comprehensive biotechnological approach to disease prevention, specially adapted to the regional conditions of southern Ukraine. The materials were processed using methods of theoretical and methodological analysis, systematic and comparative analysis, analytical and synthetic generalisation, analogy and methodological design of practical recommendations. As a result of the study, the epizootic process of coccidiosis in piglets in farms in southern Ukraine was interpreted as being sustained by a combination of the environmental reservoir of Cystoisospora suis oocysts and technologically induced repeated contamination of bedding and production surfaces. The key factors determining the invasive pressure in the holding sections are the interaction of the microclimate of the premises with the modes of moistening/ drying of substrates within the technological cycle, in particular during prolonged periods of high temperatures in the warm season and the presence of local areas of stable moisture. On this basis, prevention is described as managing the links “source of invasion – environment – susceptible contingent” with priority given to measures that reduce the persistence of invasive material between batches and limit the re-seeding of the environment. The limits of applicability of monochemical schemes in systems where the environmental component of transmission is preserved are justified, and the risks relevant to the microbiome, biosafety and environmental consequences of the prolonged presence of anticoccidial agents are outlined. A criteria-based intervention assessment scheme has been operationalised through a coordinated set of indicators reflecting parasitological, clinical and production outcomes. An integrated protocol has been proposed that combines sanitary and hygienic contamination control, biotechnological interventions, and standardised monitoring based on the “before/after” principle. The practical significance lies in the possibility of implementing a unified algorithm for prevention and control in farms in southern Ukraine with reproducible performance evaluation and comparability of effects between sections and production cycles 

Keywords:

epizootic pressure; vaccination; mucosal immunity; probiotics; antibiotic resistance

References

  1. Agazzi, A., et al. (2020). Dietary mannan oligosaccharides modulate gut inflammatory response and improve duodenal villi height in post-weaning piglets improving feed efficiency. Animals, 10(8), article number 1283. doi: 10.3390/ani10081283.
  2. Alam, M., Basir, M.S., Sultan, M.B., Murshed, M.F., Hossain, S., & Anik, A.H. (2025). Ecological footprint of ionophores in livestock production: Environmental pathways and effects. Water Environment Research, 97(3), article number e70052. doi: 10.1002/wer.70052.
  3. Albanese, G.A., Tensa, L.R., Aston, E.J., Hilt, D.A., & Jordan, B.J. (2018). Evaluation of a coccidia vaccine using spray and gel applications. Poultry Science, 97(5), 1544-1553. doi: 10.3382/ps/pey011.
  4. Araújo, H.G., Silva, J.T., Sarmento, W.F., Silva, S.D., Bezerra, R.A., Azevedo, S.S., & Vilela, V.L. (2020). Diversity of enteric coccidia in pigs from the Paraíba Semiarid Region of Northeastern Brazil. Revista Brasileira de Parasitologia Veterinária, 29(4), article number e009120. doi: 10.1590/S1984-29612020079.
  5. Arutkumaran, S., Shanmathi, S., & Hemavathi, A. (2025). The role of vaccination in controlling swine zoonosis. In R. Deb, J. Nayak, G. Singh Sengar & V. Kumar Gupta (Eds.), Emerging zoonotic threats from swine: A public health perspective (pp. 307-333). Singapore: Springer. doi: 10.1007/978-981-96-7407-7_17.
  6. Baker, D.G., Bryant, J.D., Urban Jr, J.F., & Lunney, J.K. (1994). Swine immunity to selected parasites. Veterinary Immunology and Immunopathology, 43(1-3), 127-133. doi: 10.1016/0165-2427(94)90128-7.
  7. Begma, N.A. (2023). Growth dynamics of fattening piglets under the influence of a probiotic preparation. In A. Kobets (Ed.), Theoretical and practical issues of agricultural science (pp. 401-412). Dnipro: LIRA.
  8. Bohach, O., Bogach, M., Panikar, I., Antipov, A., & Goncharenko, V. (2023). Prevalence of intestinal protozoa in pigs of Northern Black Sea Region, Ukraine. World’s Veterinary Journal, 13(2), 310-317. doi: 10.54203/ scil.2023.wvj33.
  9. Bohach, O.M., Paliy, A.P., & Bogach, M.V. (2024). Risk factors and spread of Cystoisospora suis and Cryptosporidium suis in farms of Odesa Region. Journal for Veterinary Medicine Biotechnology and Biosafety, 10(2), 3-6. doi: 10.36016/JVMBBS-2024-10-2-1.
  10. Burrough, E.R., Gabler, N.K., & Thomson, J.R. (2025). Digestive system. In J.J. Zimmerman, E.R. Burrough, L.A. Karriker, K.J. Schwartz & J. Zhang (Eds.), Diseases of swine (pp. 273-302). London: Wiley. doi: 10.1002/9781394179466. ch15.
  11. Cai, H., et al. (2023). Effects of a complex probiotic preparation, Fengqiang Shengtai and coccidiosis vaccine on the performance and intestinal microbiota of broilers challenged with Eimeria spp. Parasites & Vectors, 16, article number 253. doi: 10.1186/s13071-023-05855-5.
  12. Cheng, Y., Horng, Y., Chen, W., Hua, K., Dybus, A., Hsiao, F.S., & Yu, Y. (2021). Development and validation the efficacy of Bacillus-based fermented products as an antibiotics alternative in domestic animals. Acta Scientiarum Polonorum Zootechnica, 20(3), 23-34. doi: 10.21005/asp.2021.20.3.03.
  13. Deak, G., González-Amador, L., Goyena, E., Cârstolovean, A.S., Risueño, J., & Berriatua, E. (2024). On the efficacy of preventive toltrazuril treatments and the diagnosis of Cystoisospora suis infections in intensively raised piglets in farms from southeast Spain. Parasitology Research, 123, article number 109. doi: 10.1007/s00436024-08127-y.
  14. Delsart, M., Fablet, C., Rose, N., Répérant, J.M., Blaga, R., Dufour, B., & Pol, F. (2022). Descriptive epidemiology of the main internal parasites on alternative pig farms in France. Journal of Parasitology, 108(4), 306-321. doi: 10.1645/21-126.
  15. Dowling, P.M., & Baptiste, K.E. (2024). Miscellaneous antimicrobials: Ionophores, nitrofurans, nitroimidazoles, rifamycins, and others. In P.M. Dowling, J.F. Prescott & K.E. Baptiste (Eds.), Antimicrobial therapy in veterinary medicine (pp. 345-369). London: Wiley. doi: 10.1002/9781119654629.ch18.
  16. Dubey, J.P., & Santín, M. (2025). Coccidia and other protozoa. In J.J. Zimmerman, E.R. Burrough, L.A. Karriker, K.J. Schwartz & J. Zhang (Eds.), Diseases of swine (pp. 1161-1178). London: Wiley. doi: 10.1002/9781394179466.ch60.
  17. Fay, R., Gamelon, M., Baubet, E., & Porphyre, T. (2025). The time of infection matters: Seasonal interplay between host and pathogen drives epidemic dynamics. Oikos, 2025(3), article number e10750. doi: 10.1111/oik.10750.
  18. Gómez-Osorio, L.M., Penagos-Tabares, F., Bosnjak-Neumuller, J., Guedes, R.M., Vasiljevic, M., Steiner, T., & McOrist, S. (2025). Porcine proliferative enteropathy: Overview of disease dynamics and non-antibiotic alternatives for prevention and control strategies. Frontiers in Veterinary Science, 12, article number 1596316. doi: 10.3389/ fvets.2025.1596316.
  19. Han, H., Dong, H., Zhao, Q., Zhu, S., & Huang, B. (2024). Coccidia species and geographical distribution in genus sus: A scoping review. Microorganisms, 13(1), article number 14. doi: 10.3390/microorganisms13010014.
  20. Jankowska-Mąkosa, A., Knecht, D., Wyrembak, S., & Zwyrzykowska-Wodzińska, A. (2023). Evaluation of the level of parasites infection in pigs as an element of sustainable pig production. Sustainability, 15(4), article number 3671. doi: 10.3390/su15043671.
  21. Joachim, A., & Shrestha, A. (2019). Coccidiosis of pigs. In J.P. Dubey (Ed.), Coccidiosis in livestock, poultry, companion animals, and humans (pp. 125-145). Boca Raton: CRC Press. doi: 10.1201/9780429294105.
  22. Kato, H. (2020). Mucosal vaccine for parasitic infections. In H. Kiyono & D.W. Pascual (Eds.), Mucosal vaccines: Innovation for preventing infectious diseases (pp. 841-854). London: Academic Press. doi: 10.1016/B978-0-12811924-2.00050-X.
  23. Klikin, I., Yanovskaya, O.V., & Gordienko, Yu. A. (2024). Microbiological, biochemical, and immunological indicators when using dietary supplements in feeding pigs during growth. In A. Kobets (Ed.), All-Ukrainian scientific and practical conference of veterinarians and higher education students “From diagnosis to treatment: New horizons” (p. 36-37). Dnipro: Dnipro State Agrarian and Economic University.
  24. Kolechko, A.V., Chudak, R.A., & Shpakovska, G.I. (2023). The effectiveness of probiotic preparations in animal husbandry. Vinnytsia: Druk.
  25. Labussière, E., Achard, C., Dubois, S., Combes, S., Castex, M., & Renaudeau, D. (2022). Saccharomyces cerevisiae boulardii CNCM I-1079 supplementation in finishing male pigs helps to cope with heat stress through feeding behaviour and gut microbiota modulation. British Journal of Nutrition, 127(3), 353-368. doi: 10.1017/ S0007114521001756.
  26. Lara, A.A., Bregonde, R.B., Candeias, A.P., Dahm, V., Alberton, G.C., dos Santos, A.L., & Osaki, S.C. (2022). Evaluation of different transmission routes of Cystoisospora suis in a farm using prophylactic toltrazuril. Semina: Ciências Agrárias, 43(4), 1695-1704. doi: 10.5433/1679-0359.2022v43n4p1695.
  27. Lee, A., Stanley, J.S., Mellits, K.H., & Connerton, I.F. (2025). Prebiotic galacto-oligosaccharide and xylooligosaccharide feeds in pig production: Microbiota manipulation, pathogen suppression, gut architecture and immunomodulatory effects. Applied Microbiology, 5(2), article number 42. doi: 10.3390/applmicrobiol5020042.
  28. Lee, Y., Lu, M., & Lillehoj, H.S. (2022). Coccidiosis: Recent progress in host immunity and alternatives to antibiotic strategies. Vaccines, 10(2), article number 215. doi: 10.3390/vaccines10020215.
  29. Lillehoj, H.S., & Lillehoj, E.P. (2000). Avian coccidiosis. A review of acquired intestinal immunity and vaccination strategies. Avian Diseases, 44(2), 408-425. doi: 10.2307/1592556.
  30. Loesing, H., Bartelt, S., Cvjetkovic, V., Soeckler-Lionetti, C., Bechmann, L., Kipschull, K., Blondel, T., Mills, J., Guerra, N., & Sperling, D. (2025). Assessment of sponge sampling for real-time PCR detection of Cystoisospora suis from environmental and faecal samples from piglet-producing farms. Porcine Health Management, 11, article number 43. doi: 10.1186/s40813-025-00454-5.
  31. Memon, F.U., Yang, Y., Zhang, G., Leghari, I.H., Lv, F., Wang, Y., Laghari, F., Khushk, F., & Si, H. (2022). Chicken gut microbiota responses to dietary Bacillus subtilis probiotic in the presence and absence of Eimeria infection. Microorganisms, 10(8), article number 1548. doi: 10.3390/microorganisms10081548.
  32. Nunes, T., Skampardonis, V., Costa, F., Da Conceição, M.A., & Sperling, D. (2023). Cystoisospora suis in Portugal: An observational study of prevalence, management, and risk factors. Porcine Health Management, 9, article number 34. doi: 10.1186/s40813-023-00328-8.
  33. Palkumbura, P.A., Mahakapuge, T.A., Wijesundera, R.K., Wijewardana, V., Kangethe, R.T., & Rajapakse, R.J. (2024). Mucosal immunity of major gastrointestinal nematode infections in small ruminants can be harnessed to develop new prevention strategies. International Journal of Molecular Sciences, 25(3), article number 1409. doi: 10.3390/ijms25031409.
  34. Sander, V.A., Sánchez López, E.F., Mendoza Morales, L., Ramos Duarte, V.A., Corigliano, M.G., & Clemente, M. (2020). Use of veterinary vaccines for livestock as a strategy to control foodborne parasitic diseases. Frontiers in Cellular and Infection Microbiology, 10, article number 288. doi: 10.3389/fcimb.2020.00288.
  35. Senanayake, N.S., Boyle, L., O’Driscoll, K., Menant, O., & Butler, F. (2025). Effects of season, age and parasite management practices on gastro-intestinal parasites in pigs kept outdoors in Ireland. Irish Veterinary Journal, 78(1), article number 12. doi: 10.1186/s13620-025-00297-0.
  36. Shrestha, A., Abd-Elfattah, A., Freudenschuss, B., Hinney, B., Palmieri, N., Ruttkowski, B., & Joachim, A. (2015). Cystoisospora suis – a model of mammalian cystoisosporosis. Frontiers in Veterinary Science, 2, article number 68. doi: 10.3389/fvets.2015.00068.
  37. Simjee, S., & Tice, G. (2023). The risk-benefit balance of resistance to ionophores in Enterococcus faecium and Enterococcus faecalis for ionophore coccidiostats in broiler chickens. Journal of Antimicrobial Chemotherapy, 78, 2121-2130. doi: 10.1093/jac/dkad183.
  38. Skampardonis, V., Sotiraki, S., Kostoulas, P., & Leontides, L. (2010). Effect of toltrazuril treatment in nursing piglets naturally infected with Isospora suis. Veterinary Parasitology, 172(1-2), 46-52. doi: 10.1016/j.vetpar.2010.04.020.
  39. Straberg, E., & Daugschies, A. (2007). Control of piglet coccidiosis by chemical disinfection with a cresol-based product (Neopredisan 135-1®). Parasitology Research, 101(3), 599-604. doi: 10.1007/s00436-007-0521-z.
  40. Thong, H.T., & Duc, H.V. (2022). Potential substitutes of antibiotics for swine and poultry production. In A.A. Kamboh (Ed.), Antibiotics and probiotics in animal food-impact and regulation. London: IntechOpen. doi: 10.5772/intechopen.106081.
  41. Wang, F., Cao, L., Wang, L., Xu, J., Tao, J., & Liu, D. (2025). Characterization of the antigenic and immunogenic properties of the gametocyte antigen 56 from Eimeria necatrix. Animals, 15(12), article number 1750. doi: 10.3390/ani15121750.
  42. Youssef, I.M., et al. (2024). Mannan oligosaccharides as a prebiotic for laying hens: effects on fertility, hatchability, productive performance, and immunity. Translational Animal Science, 8, article number txae123. doi: 10.1093/ tas/txae123.
  43. Zifan, C., Chaojun, Z., Qiaoli, P., Qingfeng, Z., Yunping, D., & Huihua, Z. (2023). Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia. Poultry Science, 102(8), article number 102780. doi: 10.1016/j.psj.2023.102780.
  44.  
Share
Facebook
Twitter
LinkedIn
Email
Telegram
Viber
WhatsApp

Address
54020, Ukraine, Mykolaiv,
9 Georgiy Gongadze Str.


Email
ubsras@bsagriculture.com.ua

Main information
  • Aims and Scope
  • Indexing
  • Terms of Publication
  • Editorial Board
  • Publication Ethics
Additional information
  • Complaints Policy
  • Peer Review Process
  • Open Access Policy
  • Academic Integrity Generative AI Policy
  • Archiving